Cell wall integrity, genotoxic injury and PCD dynamics in alfalfa saponin-treated white poplar cells highlight a complex link between molecule structure and activity.
Identifieur interne : 001E62 ( Main/Exploration ); précédent : 001E61; suivant : 001E63Cell wall integrity, genotoxic injury and PCD dynamics in alfalfa saponin-treated white poplar cells highlight a complex link between molecule structure and activity.
Auteurs : Stefania Paparella [Italie] ; Aldo Tava [Italie] ; Pinarosa Avato [Italie] ; Elisa Biazzi [Italie] ; Anca Macovei [Italie] ; Marco Biggiogera [Italie] ; Daniela Carbonera [Italie] ; Alma Balestrazzi [Italie]Source :
- Phytochemistry [ 1873-3700 ] ; 2015.
Descripteurs français
- KwdFr :
- Altération de l'ADN (physiologie), Medicago sativa (composition chimique), Monoxyde d'azote (métabolisme), Paroi cellulaire (composition chimique), Paroi cellulaire (effets des médicaments et des substances chimiques), Populus (effets des médicaments et des substances chimiques), Relation structure-activité (MeSH), Résonance magnétique nucléaire biomoléculaire (MeSH), Sapogénines (composition chimique), Sapogénines (isolement et purification), Sapogénines (pharmacologie), Saponines (composition chimique), Saponines (isolement et purification), Saponines (pharmacologie), Structure moléculaire (MeSH), Triterpènes (pharmacologie).
- MESH :
- composition chimique : Medicago sativa, Paroi cellulaire, Sapogénines, Saponines.
- effets des médicaments et des substances chimiques : Paroi cellulaire, Populus.
- isolement et purification : Sapogénines, Saponines.
- métabolisme : Monoxyde d'azote.
- pharmacologie : Sapogénines, Saponines, Triterpènes.
- physiologie : Altération de l'ADN.
- Relation structure-activité, Résonance magnétique nucléaire biomoléculaire, Structure moléculaire.
English descriptors
- KwdEn :
- Cell Wall (chemistry), Cell Wall (drug effects), DNA Damage (physiology), Medicago sativa (chemistry), Molecular Structure (MeSH), Nitric Oxide (metabolism), Nuclear Magnetic Resonance, Biomolecular (MeSH), Populus (drug effects), Sapogenins (chemistry), Sapogenins (isolation & purification), Sapogenins (pharmacology), Saponins (chemistry), Saponins (isolation & purification), Saponins (pharmacology), Structure-Activity Relationship (MeSH), Triterpenes (pharmacology).
- MESH :
- chemical , chemistry : Sapogenins, Saponins.
- chemical , isolation & purification : Sapogenins, Saponins.
- chemical , metabolism : Nitric Oxide.
- chemistry : Cell Wall, Medicago sativa.
- drug effects : Cell Wall, Populus.
- chemical , pharmacology : Sapogenins, Saponins, Triterpenes.
- physiology : DNA Damage.
- Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Structure-Activity Relationship.
Abstract
In the present work, eleven saponins and three sapogenins purified from Medicago sativa were tested for their cytotoxicity against highly proliferating white poplar (Populus alba L.) cell suspension cultures. After preliminary screening, four saponins with different structural features in terms of aglycone moieties and sugar chains (saponin 3, a bidesmoside of hederagenin; saponins 4 and 5, monodesmoside and bidesmoside of medicagenic acid respectively, and saponin 10, a bidesmoside of zanhic acid) and different cytotoxicity were selected and used for further investigation on their structure-activity relationship. Transmission Electron Microscopy (TEM) analyses provided for the first time evidence of the effects exerted by saponins on plant cell wall integrity. Exposure to saponin 3 and saponin 10 resulted into disorganization of the outer wall layer and the effect was even more pronounced in white poplar cells treated with the two medicagenic acid derivatives, saponins 4 and 5. Oxidative burst and nitric oxide accumulation were common hallmarks of the response of white poplar cells to saponins. When DNA damage accumulation and DNA repair profiles were evaluated by Single Cell Gel Electrophoresis, induction of single and double strand breaks followed by effective repair was observed within 24h. The reported data are discussed in view of the current issues dealing with saponin structure-activity relationship.
DOI: 10.1016/j.phytochem.2015.01.008
PubMed: 25660272
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: stefania.paparella01@ateneopv.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Tava, Aldo" sort="Tava, Aldo" uniqKey="Tava A" first="Aldo" last="Tava">Aldo Tava</name><affiliation wicri:level="1"><nlm:affiliation>Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 29600 Lodi, Italy. Electronic address: aldo.tava@entecra.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 29600 Lodi</wicri:regionArea><wicri:noRegion>29600 Lodi</wicri:noRegion></affiliation></author><author><name sortKey="Avato, Pinarosa" sort="Avato, Pinarosa" uniqKey="Avato P" first="Pinarosa" last="Avato">Pinarosa Avato</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70125 Bari, Italy. Electronic address: pinarosa.avato@uniba.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70125 Bari</wicri:regionArea><wicri:noRegion>70125 Bari</wicri:noRegion></affiliation></author><author><name sortKey="Biazzi, Elisa" sort="Biazzi, Elisa" uniqKey="Biazzi E" first="Elisa" last="Biazzi">Elisa Biazzi</name><affiliation wicri:level="1"><nlm:affiliation>Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 29600 Lodi, Italy. Electronic address: elisa.biazzi@entecra.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 29600 Lodi</wicri:regionArea><wicri:noRegion>29600 Lodi</wicri:noRegion></affiliation></author><author><name sortKey="Macovei, Anca" sort="Macovei, Anca" uniqKey="Macovei A" first="Anca" last="Macovei">Anca Macovei</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: a.macovei@irri.org.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Biggiogera, Marco" sort="Biggiogera, Marco" uniqKey="Biggiogera M" first="Marco" last="Biggiogera">Marco Biggiogera</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: marco.biggiogera@unipv.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: daniela.carbonera@unipv.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name><affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: alma.balestrazzi@unipv.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author></analytic><series><title level="j">Phytochemistry</title><idno type="eISSN">1873-3700</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (chemistry)</term><term>Cell Wall (drug effects)</term><term>DNA Damage (physiology)</term><term>Medicago sativa (chemistry)</term><term>Molecular Structure (MeSH)</term><term>Nitric Oxide (metabolism)</term><term>Nuclear Magnetic Resonance, Biomolecular (MeSH)</term><term>Populus (drug effects)</term><term>Sapogenins (chemistry)</term><term>Sapogenins (isolation & purification)</term><term>Sapogenins (pharmacology)</term><term>Saponins (chemistry)</term><term>Saponins (isolation & purification)</term><term>Saponins (pharmacology)</term><term>Structure-Activity Relationship (MeSH)</term><term>Triterpenes (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Altération de l'ADN (physiologie)</term><term>Medicago sativa (composition chimique)</term><term>Monoxyde d'azote (métabolisme)</term><term>Paroi cellulaire (composition chimique)</term><term>Paroi cellulaire (effets des médicaments et des substances chimiques)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Relation structure-activité (MeSH)</term><term>Résonance magnétique nucléaire biomoléculaire (MeSH)</term><term>Sapogénines (composition chimique)</term><term>Sapogénines (isolement et purification)</term><term>Sapogénines (pharmacologie)</term><term>Saponines (composition chimique)</term><term>Saponines (isolement et purification)</term><term>Saponines (pharmacologie)</term><term>Structure moléculaire (MeSH)</term><term>Triterpènes (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Sapogenins</term><term>Saponins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Sapogenins</term><term>Saponins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitric Oxide</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Medicago sativa</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Medicago sativa</term><term>Paroi cellulaire</term><term>Sapogénines</term><term>Saponines</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Wall</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Paroi cellulaire</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sapogénines</term><term>Saponines</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Monoxyde d'azote</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Sapogénines</term><term>Saponines</term><term>Triterpènes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Sapogenins</term><term>Saponins</term><term>Triterpenes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Altération de l'ADN</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>DNA Damage</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Structure</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Relation structure-activité</term><term>Résonance magnétique nucléaire biomoléculaire</term><term>Structure moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the present work, eleven saponins and three sapogenins purified from Medicago sativa were tested for their cytotoxicity against highly proliferating white poplar (Populus alba L.) cell suspension cultures. After preliminary screening, four saponins with different structural features in terms of aglycone moieties and sugar chains (saponin 3, a bidesmoside of hederagenin; saponins 4 and 5, monodesmoside and bidesmoside of medicagenic acid respectively, and saponin 10, a bidesmoside of zanhic acid) and different cytotoxicity were selected and used for further investigation on their structure-activity relationship. Transmission Electron Microscopy (TEM) analyses provided for the first time evidence of the effects exerted by saponins on plant cell wall integrity. Exposure to saponin 3 and saponin 10 resulted into disorganization of the outer wall layer and the effect was even more pronounced in white poplar cells treated with the two medicagenic acid derivatives, saponins 4 and 5. Oxidative burst and nitric oxide accumulation were common hallmarks of the response of white poplar cells to saponins. When DNA damage accumulation and DNA repair profiles were evaluated by Single Cell Gel Electrophoresis, induction of single and double strand breaks followed by effective repair was observed within 24h. The reported data are discussed in view of the current issues dealing with saponin structure-activity relationship. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25660272</PMID><DateCompleted><Year>2015</Year><Month>05</Month><Day>28</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3700</ISSN><JournalIssue CitedMedium="Internet"><Volume>111</Volume><PubDate><Year>2015</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Cell wall integrity, genotoxic injury and PCD dynamics in alfalfa saponin-treated white poplar cells highlight a complex link between molecule structure and activity.</ArticleTitle><Pagination><MedlinePgn>114-23</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.phytochem.2015.01.008</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0031-9422(15)00043-6</ELocationID><Abstract><AbstractText>In the present work, eleven saponins and three sapogenins purified from Medicago sativa were tested for their cytotoxicity against highly proliferating white poplar (Populus alba L.) cell suspension cultures. After preliminary screening, four saponins with different structural features in terms of aglycone moieties and sugar chains (saponin 3, a bidesmoside of hederagenin; saponins 4 and 5, monodesmoside and bidesmoside of medicagenic acid respectively, and saponin 10, a bidesmoside of zanhic acid) and different cytotoxicity were selected and used for further investigation on their structure-activity relationship. Transmission Electron Microscopy (TEM) analyses provided for the first time evidence of the effects exerted by saponins on plant cell wall integrity. Exposure to saponin 3 and saponin 10 resulted into disorganization of the outer wall layer and the effect was even more pronounced in white poplar cells treated with the two medicagenic acid derivatives, saponins 4 and 5. Oxidative burst and nitric oxide accumulation were common hallmarks of the response of white poplar cells to saponins. When DNA damage accumulation and DNA repair profiles were evaluated by Single Cell Gel Electrophoresis, induction of single and double strand breaks followed by effective repair was observed within 24h. The reported data are discussed in view of the current issues dealing with saponin structure-activity relationship. </AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Paparella</LastName><ForeName>Stefania</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: stefania.paparella01@ateneopv.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tava</LastName><ForeName>Aldo</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 29600 Lodi, Italy. Electronic address: aldo.tava@entecra.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Avato</LastName><ForeName>Pinarosa</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70125 Bari, Italy. Electronic address: pinarosa.avato@uniba.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Biazzi</LastName><ForeName>Elisa</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 29600 Lodi, Italy. Electronic address: elisa.biazzi@entecra.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Macovei</LastName><ForeName>Anca</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: a.macovei@irri.org.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Biggiogera</LastName><ForeName>Marco</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: marco.biggiogera@unipv.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carbonera</LastName><ForeName>Daniela</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: daniela.carbonera@unipv.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Balestrazzi</LastName><ForeName>Alma</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia e Biotecnologie 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy. Electronic address: alma.balestrazzi@unipv.it.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>02</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012502">Sapogenins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012503">Saponins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014315">Triterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>31C4KY9ESH</RegistryNumber><NameOfSubstance UI="D009569">Nitric Oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>7X05537I17</RegistryNumber><NameOfSubstance UI="C005982">medicagenic acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004249" MajorTopicYN="N">DNA Damage</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000455" MajorTopicYN="N">Medicago sativa</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009569" MajorTopicYN="N">Nitric Oxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019906" MajorTopicYN="N">Nuclear Magnetic Resonance, Biomolecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012502" MajorTopicYN="N">Sapogenins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012503" MajorTopicYN="N">Saponins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013329" MajorTopicYN="N">Structure-Activity Relationship</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014315" MajorTopicYN="N">Triterpenes</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cell wall</Keyword><Keyword MajorTopicYN="N">DNA damage response</Keyword><Keyword MajorTopicYN="N">Medicago sativa</Keyword><Keyword MajorTopicYN="N">Populus alba</Keyword><Keyword MajorTopicYN="N">Programmed cell death</Keyword><Keyword MajorTopicYN="N">Saponins</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>10</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>01</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>01</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>2</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>5</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25660272</ArticleId><ArticleId IdType="pii">S0031-9422(15)00043-6</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2015.01.008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Paparella, Stefania" sort="Paparella, Stefania" uniqKey="Paparella S" first="Stefania" last="Paparella">Stefania Paparella</name></noRegion><name sortKey="Avato, Pinarosa" sort="Avato, Pinarosa" uniqKey="Avato P" first="Pinarosa" last="Avato">Pinarosa Avato</name><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name><name sortKey="Biazzi, Elisa" sort="Biazzi, Elisa" uniqKey="Biazzi E" first="Elisa" last="Biazzi">Elisa Biazzi</name><name sortKey="Biggiogera, Marco" sort="Biggiogera, Marco" uniqKey="Biggiogera M" first="Marco" last="Biggiogera">Marco Biggiogera</name><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name><name sortKey="Macovei, Anca" sort="Macovei, Anca" uniqKey="Macovei A" first="Anca" last="Macovei">Anca Macovei</name><name sortKey="Tava, Aldo" sort="Tava, Aldo" uniqKey="Tava A" first="Aldo" last="Tava">Aldo Tava</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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